Speaker
Mr
Stanislav Hrivnak
(P.J.Safarik University, Kosice)
Description
We present an alternative method for 3D tomographic imaging of microscopic biological objects in hard X-ray regime, based on Bragg Magnifier (BM) principle. BM microscope uses crystals to magnify X-ray beam resulting in advantages such as shorter propagation distances and increased dose efficiency, while achieving decent spatial resolution. This work focuses on the 3D reconstruction method to interpret the X-ray tomographic holograms using single-distance phase retrieval algorithm developed specifically for Bragg Magnifier, which is followed by filtered back-projection. We have adapted the contrast transfer function approaches for our purposes and in combination with iterative constraint-based phase retrieval algorithm we obtained faster and more robust reconstruction method. Our algorithm was successfully applied to both synthetic and real-world experimentally measured holograms of biological specimen. We reached isotropic 3D spatial resolution of 300 nm approaching theoretical resolution limit for the given experimental setup.
Primary author
Mr
Stanislav Hrivnak
(P.J.Safarik University, Kosice)
Co-authors
Mr
Andrej Hovan
(P.J.Safarik University, Kosice, Slovakia)
Prof.
Jozef Ulicny
(P.J.Safarik University, Kosice, Slovakia)
Dr
Patrik Vagovic
(European XFEL, Hamburg)
