Mr
Stepan Osipov
(Moscow Institute of Physics and Technology)
F-type ATP synthase is a large protein complex that synthesizes ATP from ADP and phosphate. It plays a key role in the bioenergetics of any organism. There are three classes of F-type ATP synthase: bacterial, chloroplast, and mitochondrial. Despite significant structural similarities, there are also significant differences between different ATP synthases. Specifically, mitochondrial ATP synthases function as dimers or higher-order oligomers, while chloroplast ATP synthases typically exist as monomers, except for a small ~15% oligomeric fraction.
We have isolated and purified ATP synthase from spinach chloroplasts and investigated its in vitro dimerization process. Structural data obtained using SAXS indicate the dimerization of ATP synthase depending on the ionic strength of the solution. The model that best describes the SAXS data is an I-shape dimer with contacts through the delta subunit. The possible physiological role of chloroplast ATP synthase dimerization is discussed.
We acknowledge the support from the Russian Science Foundation (RSF) Project 22-74-00044.
Mr
Stepan Osipov
(Moscow Institute of Physics and Technology)
Yury Ryzhykau
(Moscow Institute of Physics and Technology)
Ms
Andronika Minaeva
(Research Center for Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology)
Egor Zinovev
(Moscow Institute of Physics and Technology)
Sergey Ivashchenko
(Research Center for Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology)
Mr
Dmitry Verteletskiy
(Research Center for Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology)
Vsevolod Sudarev
Daria Kuklina
(MIPT)
Mikhail Nikolaev
(MIPT)
Mr
Yuriy Semenov
(Research Center for Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology)
Dr
Yuliya Zagryadskaya
(Research Center for Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology)
Dr
Ivan Okhrimenko
(Research Center for Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology)
Margarita Gette
(Moscow Institute of Physics and Technology)
Elizaveta Dronova
(Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, Dolgoprudny, 141700, Russian Federation)
Mr
Aleksei Shishkin
(Research Center for Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology)
Dr
Norbert Dencher
(Research Center for Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology)
Alexander Kuklin
(JINR)
Dr
Vladimir Uversky
(Department of Molecular Medicine and Byrd Alzheimer’s Research Institute , Morsani College of Medicine, University of South Florida, Tampa, FL 33620 , USA)
Alexey Vlasov
(MIPT)
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