The consumption of wild and farmed mussels collected from the coastal water areas with different levels of anthropogenic pressure could lead to harm for human health. The safety of mussels as food could be assessed according to level of heavy metals and other toxic microelements in the soft tissues. In the study the risks of mussel consumption for local populations were assessed by using three...
Neutrophils release decondensed chromatin or extracellular traps (NETs) in response to various physiological and pharmacological stimuli. Apart from host defensive functions, NETs play an essential role in the pathogenesis of various autoimmune, inflammatory, and malignant diseases. In recent years, studies have been performed on photo-induced NET formation, mainly activated by UV radiation....
Transglutaminase 2 (TG2) is a pivotal enzyme involved in various biological processes such as wound healing, apoptosis, and cell differentiation. Depending on the environmental conditions, TG2 can adopt two distinct conformations: the open and closed states. Notably, the open conformation of TG2 has been associated with the pathogenesis of several diseases, including celiac disease and certain...
The boron neutron capture therapy technique has been appealing to advance the technical and medical development of aspects of malignant tumors. The concept of the method has been aimed at dose contribution by secondary particles for targeted tumor sites while neutron beams do not have enough radiation effects to damage healthy cells with an advantage. This study focused on calculating the dose...
The patterns of induction and repair of clustered DNA double-strand breaks (DSBs) (DSBs) in normal human skin fibroblasts under the action of γ-rays 60Co, protons, accelerated ions 11B, 20Ne, 15N and 12C were investigated. Using immunocytochemical staining method, radiation-induced γH2AX/53BP1 foci were analyzed in detail in 3D images.
It was found that the action of accelerated heavy...
The key damage to the cell that determines its fate is double-strand breaks (DSB) of DNA, the incorrect repair of which leads to chromosomal aberrations that can be fatal for the cell [1]. Therefore, understanding the mechanisms of induction and kinetics of DSBs DNA repair is important for predicting the radiation-induced response to radiation therapy and the survival of cells that have been...
It is known, that about 8% of the total land area is irrigated by wastewater, and vegetables are among the main crops produced via wastewater irrigation throughout the world. Generally, industrial wastewaters are contaminated with an appreciable amount of trace and toxic elements, that can be accumulated in agricultural soils and crops and then transferred to the human body via the food chain...
A study of ketoprofen was carried out using experimental as well as computational methods. Ketoprofen is a representative of the group of non-steroidal anti-inflammatory drugs widely used in modern medical therapy. It has a high analgesic, anti-inflammatory and antipyretic activity. Reactivity of ketoprofen is closely connected with its structures features. Ketoprofen sample was investigated...
The main purpose of this study is to investigate the physicochemical properties of a pharmaceutical substance called ibuprofen. The research of medicines is crucial for the promotion and improvement of the healthcare system. The first aim of the study is to check the purity of the ibuprofen sample obtained in the pharmacy and its analysis in comparison with the information provided in the...
Methods of X-ray diffraction analysis (XRD) have allowed us to determine the structure of many materials and substances around us. The configurations of crystal lattices of a variety of inorganic materials became known to science, which made it possible to create new compounds with specified properties and characteristics. Advances in the decoding of solid materials and crystals were attempted...
New recombinant vaccines based on globular protein complexes with fused immunogens have shown amazing efficiency against a number of diseases, e.g., influenza, HIV, HCV, Epstein-Barr, etc. The features of such vaccines: multiple antigen representation, assembly of trimeric viral surface proteins on the surface of 24-meric protein apoferritin at the places of its three-fold channels, and a dual...
Ferritin is a globular protein complex essential for iron assimilation and storage within the protein shell of apoferritin. Given its unique biochemical properties and globule topology, ferritin has diverse applications, some of which are already realized, while others remain prospective. This work focuses on a potential application of ferritin as a dietary supplement for iron deficiency and...
The unique Dsup protein discovered in the tardigrade Ramazzottius varieornatus has been shown to increase resistance to radiation and oxidative stress in various model organisms, including human cell culture. The hypothesis suggests that Dsup forms a complex with DNA, which reduces damage caused by reactive oxygen species formed during water radiolysis. In order to understand the molecular...
At the present time there is a hypothesis about the key role of amyloid beta peptide in the onset of Alzheimer's disease. It is considered that its interaction with cell membranes causes a disruption of their permeability and integrity, which may trigger further neurodegenerative processes. The experimental study showed that the peptide takes part in the morphological changes of the...
During surgical intervention it is important to know the state of the sutured tissues to prevent postoperative complications. One of the possible ways to determine tissue condition is to measure the level of the oxygen saturation. In this work considered goal was reached by the method of diffuse scattering spectroscopy. The equipment, which was used to perform the measurements, included...
Studies of new proteorhodopsins from various bacteria have shown that they have both similarities and differences in their functional properties compared to the model rhodopsin - BR. In particular, the group of microbial rhodopsins with the DTG motif in the active center, which includes the proteorhodopsin from Sphingomonas paucimobilis (SpaR), may be of interest [Malyar N., 2020].
In...
ATP synthase is an essential protein complex integral to the bioenergetics of all living organisms. Located within the membrane, it facilitates the conversion of ADP into ATP using an inorganic phosphate, leveraging the proton concentration gradient across the membrane [1]. The proton flow induces rotation in the membrane domain of ATP synthase (specifically, the rotor c-ring). This rotation...
Ferritin is a protein complex whose presence is vital in almost all living organisms due to its responsible for storing iron. The protein is essential to iron homeostasis and is involved in a wide range of physiologic and pathologic processes. According to [1], ferritin’s mechanism of self-assembly can be used as part of an effective small molecule delivery system. Therefore, there are a lot...
Ferritin is a protein complex responsible for storing iron in various organisms. One of the most important properties of ferritin is the spontaneous formation of a spherical hollow protein globule consisting of 24 subunits, this process is called self-assembly [1]. The protein globule is very stable and can sustain significant temperature and pH changes [2]. The self-assembly process plays a...
Abstract— We report the different pulse duration of UVB laser radiation cytotoxic effect on human skin fibroblasts. The shorter the pulse, the less viable the cells. According to MTT test examined 24 hours after irradiation cells have the potential to recover. Regarding flow cytometry, we concluded that the toxicity is concerned with the lipid membrane destruction.
Vitiligo is the...
