Ionizing radiation with different physical characteristics induces a wide spectrum of DNA damage. The structural DNA damage, such as single-strand breaks (SSB), double-strand breaks (DSB) and AP sites (abasic sites missing either a pyrimidine or purine nucleotide), as well as cluster damage, which are the set of all these damage, is the main factor which could lead to the formation of various...
An issue of crew members’ health care is raised while planning long-term expeditionary space missions. It was determined that astronauts encounter an increased risk of developing bacterial infections, respiratory diseases, dermatoses and allergic reactions, gastrointestinal disorders, as well as reactivation of certain viruses during the flight to the ISS. This is in part the fact that the...
It was shown ischemic cerebral stroke stimulates neurogenesis in the subrentricular zone of the lateral ventricles and the subgranular zone of the dentate gyrus of the hippocampus. In the MCAO model of ischemic stroke, these processes have been well studied, but changes in the genetic apparatus of the cells of the neurogenic niche have not been studied. We conducted a study of telomere length...
High-order clustered DNA lesions is the hallmark of the action of dense-ionizing radiation. It is defined as a combination of two or more individual lesions (single-strand and double-strand breaks (DSB), base damage, etc.) located within 1-2 DNA helical turns. Clustered DNA DSBs, which contains DSB and other DNA lesions, represent specific interest for investigation.
For investigation of...
DNA DSBs repair kinetics in neurons and astrocytes of primary hippocampal cell culture after irradiation with Co60 g-rays and proton
Abstract
The induction and repair of DNA double-strand breaks (DNA DSB) were analyzed in primary hippocampal cell culture under the action of Co60 g-rays or protons at 3 Gy dose. For investigation of DNA DSBs formation, we established two cell cultures:...
Investigation of the effects of ionizing radiation on cells of the central nervous system is a challenging topic in modern radiation research, as well as treatment planning in radiation therapy and predicting the space radiation health risk for exploration cosmonauts. To contribute in understanding radiation damage mechanisms in complex morphology of brain cells, it is necessary to investigate...
Over the last decades, concerted efforts to decrease tobacco consumption worldwide have been made. There is an extensive body of research on the content of harmful substances in tobacco raw materials and in cigarette smoke. Data on the statistical relationships between the heavy metal content in soils and in tobacco plants are of interest. For this study, 38 soil samples and 38 samples from...
Sponges are one of the oldest multicellular animals that have survived almost unchanged to this day. The earliest finds of sponge remains date back 680 Ma. At the moment, the planet is inhabited by both marine and freshwater sponges. By the type of nutrition, all sponges are filter feeders, so they play an important role in aquatic ecosystems. At a certain moment events of mass diseases and...
